The second abnormality is enhanced nucleation of cholesterol crystals.
“Mucin and its congeners, the major proteins, act as matrix molecules to hold cholesterol crystal aggregates together to form a stone.
“There also must be sufficient time for nucleation to occur, for crystals to form and grow to microliths, and for microliths to aggregate to form gallstones, hence gallbladder stasis is a contributing factor to gallstone formation.
“During overnight fasting, the gallbladder does not empty so that hours of storage occur in all individuals.
“It should be the sum of these three factors that determines when gallstones form.”